Chlorophyll
Plants and algae conduct photosynthesis by absorbing
sunlight. Fluorescence is the re-emission of previously absorbed
light. Chlorophyll-a, the central light-absorbing pigment in
algae, is highly fluorescent.
The chlorophyll fluorescence of living algae cells (in
vivo) can be used easily to determine:
-
chlorophyll content
-
photosynthetic
activity
|
 |
The advantages of in vivo fluorescence measurement are:
- high sensitivity
- no sample preparation
-
rapid performance
-
low costs
|
 |
Interference can occur with compound such as:
- CDOM (colored-dissolved organic matter),
- yellow substances accessory algal pigments
- large amounts of turbid particles
bbe spectrofluorometers for the measurement of
micro-algae, picoplankton, nanoplankton and benthic algae:
- use the advantages of in vivo fluorescence measurement
- correct for CDOM
- determine the effects of accessory algal pigments
- calculate the chlorophyll-a of up to 5 different algae
classes
- determine the transmission
- determine the photosynthetic activity
|
 |
bbe spectrofluorometers are used for practical work in:
- drinking water monitoring
- monitoring of reservoirs & rivers
- environmental/ ecological assessment
- monitoring of lakes for bathing
- algal bloom detection
- marine research
- limnological research
- education
|
 |
bbe spectrofluorometers are for:
|
|
