Quick chlorophyll analysis in the laboratory

The instrument for the...

• quantifying of the spectral algae classes:
  green algae, blue-green algae, brown algae (diatoms and dinoflagellates) and cryptophyceae
• determination of total chlorophyll content
• determination of photosynthetically active chlorophyll

The chlorophyll analysis includes the determination of the chlorophyll content, which replaces wet-chemical analysis.

The pigments are spectrally excited by colored LED's. This enables the determination of the distribution of chlorophyll across the different algae classes. The photosynthetic activity of the algae is determined by the fluorescence pattern of the pigment excitation (Genty).

Measurements

• of direct chlorophyll fluorescence:
  is performed without sample preparation and is therefore much faster than common chlorophyll analysis; in spite of an average measuring time of only 3 minutes, the results are comparable to HPLC or wet-chemical analysis (R²>0.93) of differentiation of spectral algae classes:
• to determine the content of chlorophyll emerging from green algae, blue-green algae, brown algae (diatoms and dinoflagellates) and cryptophyceae; the pigments of different algae are determined by using coloured LEDs (included fluorescence spectrometer); adding additional algae with special pigment distribution is possible after determining a standard spectrum of the Genty parameter, as well as fm, f, f0:
• to the oxygen production rate; the values determine the percentage of photosynthetically active chlorophyll under illumination and allows the distinction between active chlorophyll and other fluorescence-emitting compounds determination of toxicity:
• by comparison of a polluted sample plus algae with untreated algae transmission:
  takes place during each analysis and, if necessary, can be used to compensate the influence of substances that cause turbidity; a sample transmission can also be determined.

Application:

Waterway analysis and assessment general environmental assessment intake monitoring chemical analysis toxicity testing waste detection dam monitoring limnological work research and education

New Algae Lab Analyzer

 

Software
The database software is used to record and analyze the data. The most important features are...

saving of data and parameters at any time graphic display of all measurement values online display in LAN calibration of the instrument parameterisation of the measurement data export to EXCEL and to text files print function subsequent recalibration of algae classes

Literature:

chlorophyll measurements

On the Web:

bbe instruments on the web

chlorophyll on the web

Specifications of the bbe AlgaeLabAnalyser

Measurands

• total chlorophyll [µg Chl-a/cm²]
• concentration of green algae [µg Chl-a/l]
• concentration of blue algae [µg Chl-a/l]
• concentration of diatoma [µg Chl-a/l]
• concentration of cryptomonades[µg Chl-a/l]
• Gelbstoffe (yellow substances)
• photosynthetic activity (Genty) - Option
• transmission (at 5 wavelengths)